EXTRACTION AND IDENTIFICATION OF MAJOR POLYPHENOL CONSTITUENTS OF NORTHERN HUNGARIAN HORSEMINT (MENTHA LONGIFOLIA L. (L.))

Authors

  • Katalin PATONAY Food and Wine Research Institute, Eszterházy Károly University, 6G Leányka u., H-3300, Eger, Hungary
  • Orsolya SZABÓ-HUDÁK Food and Wine Research Institute, Eszterházy Károly University, 6G Leányka u., H-3300, Eger, Hungary
  • Helga SZALONTAI Food and Wine Research Institute, Eszterházy Károly University, 6G Leányka u., H-3300, Eger, Hungary
  • Mihály JÁNÓSZKY Food and Wine Research Institute, Eszterházy Károly University, 6G Leányka u., H-3300, Eger, Hungary
  • Erika PÉNZESNÉ KÓNYA Department of Botany and Plant Physiology, Eszterházy Károly University, 6D Leányka u., H-3300, Eger, Hungary
  • Éva ZÁMBORINÉ NÉMETH Department of Medicinal and Aromatic Plants, Szent István University, 29-43 Villányi u., H-1118, Budapest, Hungary

DOI:

https://doi.org/10.21406/abpa.2020.8.1.53

Keywords:

Mentha longifolia, horsemint, polyphenolic antioxidants, HPLC, antioxidant activity

Abstract

(Mentha longifolia (L.) L.) is a less studied species from the viewpoint of its nonvolatile constituents and this work is the first phytochemical screening of it in Hungary. The aim of the experiments is to elaborate an efficient extraction method of its polyphenolic antioxidants. Thirty-six accessions were sampled. Soxhlet and an ultrasonic extraction were applied, both with methanol (MeOH) and ethanol:water 7:3 (WA). The analysis of the extracts was carried out by HPLCDAD method. Rosmarinic acid, hesperidin, diosmin and cynaroside were observed to be common constituents of all samples. Other major constituents were observed in all of the chromatograms at ca. tR=13.5 min. and tR=19.7 min (unknown A and B). Their identification needs further examination. Regarding antioxidant activity, WA extraction was more efficient than MeOH Soxhlet extraction.

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Published

2020-09-09